Bleeding Edge Biology

Deep-Sea Bioprospecting: Exotic Drugs & Technology from the Abyss

 

Introduction

The Uncharted Depths of Our Oceans

The oceans, covering over 70% of our planet, stand as Earth’s last great unexplored frontier. Indeed, we’ve mapped the deep sea less thoroughly than the Moon or Mars. However, the deep ocean’s extreme conditions—intense pressure, frigid temperatures, and complete darkness—drive the evolution of creatures with extraordinary characteristics and biochemical properties not found on land. Moreover, the deep sea is a treasure trove of biodiversity, with about 34 marine animal phyla identified, contrasting sharply with the roughly 15 phyla among terrestrial animals. These characteristics are driving interest in deep-sea bioprospecting.

 

The Pursuit of Deep-Sea Bioprospecting

Deep-sea bioprospecting aims to uncover, collect, and study these organisms, leveraging their unique traits for advances in medicine, industry, and technology. Specifically, in the medical field, scientists are discovering novel organisms and compounds with properties ideal for developing new antibiotics, anticancer agents, and antivirals. Beyond medical applications, the deep sea also serves as a source of innovative materials and manufacturing techniques.

 

This exploration faces significant challenges, however. The ocean’s harsh conditions complicate sample collection and necessitate advanced technologies. Moreover, the small amounts of bioactive compounds typically present demand complex extraction and amplification techniques, adding to research complexity. Despite these hurdles, the the ocean’s vast potential for deep-sea bioprospecting must be mindful of the need for sustainable exploration and global cooperation.

 

Countries around the globe are currently engaged in deep-sea bioprospecting, driven by a shared interest in the ocean’s rich biodiversity. Notably, nations like the United States, Japan, Germany, France, China, and Brazil, along with organizations such as the Scripps Institution of Oceanography, Woods Hole Oceanographic Institution, and the National Oceanography Centre in the UK, play crucial roles in deepening our understanding of these ecosystems. Furthermore, international collaborations, including the Census of Marine Life and the Ocean Biogeographic Information System, unite experts in a collective effort to explore and document the ocean’s breadth.

 

The exploration of deep-sea environments promises unparalleled scientific breakthroughs. However, it is vital that we approach deep-sea bioprospecting sustainably to protect these fragile ecosystems from harm. As interest in deep-sea bioprospecting grows, implementing stringent international maritime law and conservation efforts becomes essential to safeguard the deep sea from overexploitation and environmental damage.

 

The Deep Sea: A Mysterious World

Life in the Abyss

Barreleye Fish. An example of a deep-sea organism.
Macropinna microstoma, showing the transparent membrane protecting the eyes. Kim Reisenbichler, CC BY-SA 4.0, via Wikimedia Commons

 

Spanning depths from 200 to nearly 11,000 meters, the deep sea teems with life, ranging from the minuscule to the magnificent. Extremophiles here exhibit remarkable adaptations to withstand the deep sea’s harsh conditions. Among them, the deep-sea anglerfish, known for its glowing lure, the Dumbo Octopus with its distinctive fins, and the Barreleye, a deep-sea fish with a transparent head, demonstrate the extraordinary adaptations necessary for survival in a sunlight-deprived environment.

 

Hydrothermal Vents: Oases of the Deep

Giant tube worms. An example of a species living around deep-sea hydrothermal vents.
Giant tube worms Riftia pachyptila cluster around vents in the Galapagos Rift. NOAA Okeanos Explorer Program, Galapagos Rift Expedition 2011.

 

Furthermore, deep sea hydrothermal vents, which emit superheated, mineral-rich water, can reach temperatures exceeding 400°C (752°F). These vents support vibrant ecosystems, thriving in the complete absence of sunlight. Among the most heat-tolerant multicellular organisms found here are Pompeii worms, which closely associate with these vents. Additionally, vent snails, blind shrimp, clams, mussels, giant tube worms, deep-ocean fish known as eelpouts, and yeti crabs, form symbiotic relationships with chemosynthetic bacteria. These bacteria convert hydrogen sulfide and other inorganic molecules from the vents into organic matter, illustrating the vents’ role as deep-sea biodiversity hotspots.

 

Cold Seep Communities: A Diverse Ecosystem

Moreover, cold seeps, where hydrocarbon-rich fluids escape into the ocean, foster communities of mussels, clams, and other deep-sea fauna. Notably, the tube worm Lamellibrachia luymesi, which flourishes in cold seep environments, can grow over two meters long, with some individuals living more than 250 years. Like their counterparts at hydrothermal vents, animals in these communities engage in symbiotic relationships with chemosynthetic bacteria, showcasing the complex interdependencies characteristic of deep-sea life.

 

The Challenger Deep

Another unique ecosystem can be found in the Challenger Deep, the deepest part of the ocean located in the Mariana Trench, reaches depths of almost 7 miles. This area is home to a variety of creatures, such as amphipods, foraminifera, sea cucumbers, and polychaete worms. These organisms depend on chemosynthesis and the detritus falling from the ocean’s upper layers for energy, demonstrating the incredible adaptability of life under extreme conditions. Such adaptability often requires genetic and biochemical innovations with the potential to drive new drugs and technologies. 

 

The Challenges of Exploring Habitats for Deep-Sea Bioprospecting

Navigating the Abyss

Launching Alvin into the sea; note the sampling basket at the forward part of the sub with all of the sampling equipment attached.

 

Exploring the deep sea mirrors the challenges of venturing onto another planet. The extreme pressure, exceeding 8 tons per square inch in certain regions, are akin to the weight of 48 Boeing 747 airplanes pressing down on a single square foot. Additionally, navigating in perpetual cold and darkness further complicates exploration. Researchers rely on artificial lights from submersibles to light their way, yet these lights can only reveal a small portion of the vast, unseen world at any given time. Consequently, mapping the ocean floor becomes a slow, tedious process that demands both patience and precision.

 

Moreover, the deep sea’s isolation poses significant logistical challenges. Expeditions to these remote locales are both time-consuming and costly, necessitating ships equipped with the necessary gear to launch and recover exploration equipment. The harsh conditions further limit the duration divers and vehicles can remain underwater, thus restricting the amount of data collected during each dive.

 

The Role of Cutting-Edge Technologies in Deep-Sea Bioprospecting

Alvin, an example of an underwater submersible for exploring the deep sea.
Underwater explorer Alvin. Courtesy of NOAA.

 

Consequently, in the realm of deep-sea exploration, advanced technologies play a pivotal role. Deep-sea submersibles, engineered to withstand extreme pressures, enable researchers to directly observe the mysterious life forms of the deep sea. These vessels blend the excitement of discovery with the ability to make real-time decisions.

 

Additionally, unmanned, remote-operated vehicles (ROVs) undertake deep-sea dives, capturing detailed images and samples. These tethered robots navigate challenging terrains and perform tasks deemed too risky or intricate for humans, proving indispensable for their versatility and safety.

 

Similarly, autonomous underwater vehicles (AUVs) venture into the deep without human guidance, mapping the seafloor and capturing images of its shadowy recesses. Their capacity to independently cover vast areas renders them invaluable for data collection in regions beyond the reach of human explorers.

 

The Unyielding Pull of the Deep

Despite the array of challenges, the deep sea’s enchantment remains undiminished. Concealed beneath kilometers of water, its enigmas and potential treasures continue to captivate explorers and scientists. The pursuit of uncovering these mysteries, propelled by both the thrill of discovery and the advancement of technology, underscores the relentless human drive to explore the unknown.

 

The Race to Discover: Deep-Sea Bioprospecting Stakeholders and Motivations

Unveiling the Ocean’s Potential Through Deep-Sea Bioprospecting

In the realm of deep-sea bioprospecting, a collaborative effort unites governments, academic institutions, and private companies. Their shared objective? To delve into the ocean’s depths in search of new organisms and compounds beneficial for medicine, industry, and environmental stewardship. This cooperative venture brings together various organizations to tap into the ocean’s secrets for innovative advancements.

 

A notable deep-sea bioprospecting collaboration is between the Scripps Institution of Oceanography and the National Institutes of Health (NIH) in the US. Together, they focus on analyzing genetic material from deep-sea organisms, aiming to uncover molecules that could pave the way for novel medications.

 

Internationally, the Marine Biodiscovery Center at the University of Aberdeen is on a quest for biologically active molecules from marine invertebrates and microorganisms. Their research, which extends to deep-sea bioprospecting, targets potential treatments for conditions like cancer, bacterial/fungal infections, and inflammation.

 

Aquapharm Biodiscovery Ltd., also based in Scotland, showcases the practical side of bioprospecting. The company utilizes its vast collection of marine microorganisms, many from the deep sea, to develop bioactive compounds for use in pharmaceuticals, nutraceuticals, personal care products, and various industrial applications.

 

The Role of Ecological and Geological Studies in Deep-Sea Bioprospecting

Ecological and geological explorations are crucial for a deeper understanding of deep-sea environments. Dr. Cindy Van Dover from Duke University and Dr. Jon Copley from the University of Southampton stand out for their contributions to hydrothermal vent ecology. Their work significantly enhances our knowledge of these complex ecosystems.

 

Furthermore, a notable government initiative is that led by the Ocean Exploration Trust and backed by the U.S. National Oceanic and Atmospheric Administration (NOAA). These so-called Nautilus expeditions are headed by Dr. Robert Ballard, famed for discovering the wrecks of the Titanic and the Bismarck. They focus on exploring poorly understood ocean areas using advanced ROVs.

 

On the private sector front, Ocean Infinity embarks on the ambitious Seabed 2030 project, striving to map the entire ocean floor using AUVs and cutting-edge sonar technology. Their goal is to enhance maritime navigation, underwater archaeology, and environmental monitoring.

 

Notably, these endeavors all rely on the development of sophisticated equipment. Spearheading this issue, The Woods Hole Oceanographic Institution’s Deep Submergence Laboratory (DSL) specializes in the innovation of remote and unmanned systems for deep-sea research. DSL’s engineers and scientists equip exploration vehicles for undertaking critical surveys, observations, and sampling in the planet’s most inaccessible marine regions.

 

From Discovery to Application: The Deep-sea Bioprospecting Development Pipeline

 

Initial Deep-Sea Bioprospecting Analysis and Discovery

Once researchers bring samples to the surface, they immediately catalog and store them in conditions free of contaminants that closely mimic their natural environment. This crucial step lays the groundwork for the comprehensive lab analysis to follow, which zeroes in on the samples’ genetic and chemical makeup. Subsequently, scientists employ techniques like genomic sequencing, chromatography, and mass spectrometry to unveil new genes and identify previously unknown compounds.

 

Government-funded research agencies frequently back these initial phases of deep-sea bioprospecting, aiming to delve into the mysteries of these unique organisms and their environments. The valuable data collected, including billions of genetic sequences, find their way into public databases such as GenBank, becoming indispensable resources for biotech startups, pharmaceutical firms, and other stakeholders. Utilizing advanced bioinformatics tools, including AI, researchers sift through these databases to find therapeutic candidates for synthesis and testing.

 

While some may critique the shift from public research to commercial development, it’s important to recognize that without comprehensive government oversight of the entire research and development process, commercial entities play a pivotal role in transforming deep-sea bioprospecting discoveries into accessible innovations.

 

Challenges in Developing Products from Deep-Sea Bioprospecting

Transitioning from discovery to application is laden with hurdles that demand creativity, perseverance, and cutting-edge technological solutions. A significant challenge is the replication of the deep sea’s extreme conditions on land, such as high pressures, frigid temperatures, and the absence of light. Cultivating deep-sea organisms, therefore, often requires specialized and expensive equipment.

 

Additionally, the extraction of bioactive compounds from these organisms entails complex, multi-stage processes. Given the typically modest yields from these samples, scaling up to meet research and development needs is daunting, frequently necessitating the development of novel chemical synthesis methods.

 

Ecological and ethical considerations further complicate the pathway to commercialization. Adopting sustainable deep-sea bioprospecting practices is paramount to safeguarding these ecosystems, while regulatory hurdles aimed at conserving marine biodiversity and ensuring equitable benefit sharing can impede the speed of commercializing deep-sea-derived products.

 

Despite these formidable obstacles, the allure of discovering new medications, materials, and technologies continues to fuel exploration and innovation in deep-sea bioprospecting. This challenging yet promising frontier beckons with the potential for revolutionary breakthroughs, highlighting the urgent need for sustainable and innovative approaches in navigating this largely uncharted territory.

 

Promising Molecules Derived from Deep-Sea Bioprospecting

 

Potential Deep-Sea Bioprospecting Therapeutics

Microbial Compounds

Deep-sea microbes represent a treasure trove of novel therapeutic molecules with groundbreaking potential in medical science. For instance, a bacterium found at depths of 1100 meters, known as Salinispora tropica, produces Salinosporamide A (Marizomib). This potent proteasome inhibitor is currently in phase III clinical trials for treating glioblastoma and has the unique ability to cross the blood-brain barrier, overcoming a significant challenge in brain drug delivery.

 

Furthermore, other potential therapeutics derived from bacteria living at depths ranging from 1000 to nearly 11,000 meters include Diketopiperazines, Loloatins, and Dermacozines, all under investigation for their anticancer properties. Additionally, compounds such as Abyssomicin C, Pseudonocardians A–C, and Caboxamycin are being studied for both antibiotic and anti-tumor activities.

 

Moreover, the bacteria Marianactinospora thermotolerans, is known to produce antimalarial compounds, highlighting the vast biomedical potential lying in the depths of the ocean. Deep-sea fungi add to this rich biochemical diversity by producing metabolites with antibacterial, antifungal, anticancer, and anti-inflammatory properties.

 

Compounds from Invertebrates

Venus' Flower basket an example of an organism from the deep sea.
A spectacular group of Venus flower basket glass sponges (Euplectella aspergillum) glass sponges with a squat lobster in the middle. NOAA Okeanos Explorer Program, Gulf of Mexico 2012 Expedition.

 

 

Marine sponges, too, are prolific producers of bioactive compounds. For example, Topsentins from the deep-water sponge Spongosorites ruetzleri, Lasonolide A from Forcepia sp., and Discodermolide from Discodermia spp. have shown promise in anti-proliferative, antimicrobial, and Alzheimer’s disease therapies, respectively.

 

Additionally, the deep-sea sponge Euplectella aspergillum, or Venus’ flower basket, has inspired innovations beyond the realm of biomedicine, influencing engineering and technology with its robust lattice skeleton. This has laid the groundwork for stunning architectural designs, such as London’s Gherkin tower, ideas for optimizing fluid flow, and potentially novel processes for manufacturing silica glass fibers.

 

Lastly, complex deep-sea organisms like the Antarctic tunicate Aplidium sp., the crinoid Holopus rangii, and the mussel Bathymodiolus thermophilus have been sources of compounds like Rossinones A and B, Gymnochrome E and F, and Bathymodiolamides A and B, demonstrating their potential as cancer therapeutics.

 

Deep-Sea Enzymes for Sustainable Manufacturing and Bioremediation

Enzymes from deep-sea extremophiles could revolutionize industrial processes. Their resilience under high pressures, extreme temperatures, and varying pH levels makes them ideal for manufacturing and bioremediation, catalyzing reactions efficiently under challenging conditions.

 

Proteases, lipases, carbohydrases, and oxidoreductases have notable industrial applications. Proteases, for instance, contribute to energy savings in the detergent industry by enabling cold water washing. Similarly, lipases play a pivotal role in biodiesel production by aiding the transesterification of fats and oils. Carbohydrases, on the other hand, are crucial for converting plant biomass into fermentable sugars for bioethanol production, while oxidoreductases support environmentally friendly pharmaceutical manufacturing by facilitating the synthesis of complex drug molecules under green conditions. 

 

Furthermore, enzymes capable of polymerizing new materials could lead to the creation of polymers boasting enhanced durability and resistance to environmental degradation.

 

Additionally, in the realm of bioremediation, enzymes like oxidoreductases, hydrolases, dehalogenases, laccases, and nitrilases target specific pollutants, offering solutions for detoxifying contaminants like PCBs, dioxins, oil spills, chlorinated solvents, synthetic dyes, and cyanide-based industrial waste.

 

In sum, integrating deep-sea enzymes into various processes represents a significant advancement in leveraging nature’s ingenuity for human benefit, further underscoring the vast potential of deep-sea bioprospecting.

 

Legal and Ethical Considerations of Deep-Sea Bioprospecting

 

Legal Frameworks Governing Deep-Sea Bioprospecting

Deep-sea bioprospecting is governed by a sophisticated web of international laws, ensuring the quest for knowledge and resources does not compromise the marine environment or equitable access to its wealth.

 

Central to this legal landscape is the United Nations Convention on the Law of the Sea (UNCLOS), often hailed as the “Constitution for the Oceans.” It outlines the regulatory framework for marine activities, detailing states’ rights and responsibilities in marine exploration and exploitation. Significantly, UNCLOS enshrines the concept that the deep sea and its bounty are the “common heritage of mankind.” Accordingly, this principle demands equitable benefit-sharing from deep-sea bioprospecting, fostering a bridge between technologically advanced nations and developing countries that may lack the capabilities for such exploration.

 

Moreover, the Nagoya Protocol, augmenting the Convention on Biological Diversity (CBD), refines this framework further. It mandates the fair and equitable distribution of benefits derived from genetic resources, including those from deep-sea environments. These benefits extend beyond financial gains to include scientific training, technology transfer, and capacity-building efforts, thereby encouraging a spirit of global partnership in the exploration of deep-sea genetic riches.

 

Ethical Considerations in Deep-Sea Bioprospecting

From an ethical standpoint, deep-sea bioprospecting raises significant questions about stewardship over Earth’s final frontiers. The precautionary principle, a foundational tenet of environmental ethics, calls for prudence in bioprospecting endeavors. It insists on comprehensive evaluations of potential environmental impacts before the initiation of exploration and exploitation activities, striving for a harmony between discovery ambitions and the imperative to conserve marine biodiversity and ecosystem integrity.

 

Furthermore, the ethical dimension of bioprospecting extends to acknowledging and integrating the knowledge of indigenous and local communities. Their ancestral wisdom can offer invaluable perspectives on marine biodiversity, making it imperative to ensure their recognition, respect, and fair compensation for their contributions to ethical bioprospecting efforts.

 

In summary, navigating the complex legal and ethical terrains of deep-sea bioprospecting demands a delicate balance between exploratory zeal and environmental stewardship. Such an approach guarantees that the treasures of the deep sea enrich humanity at large while safeguarding these wonders for the enjoyment and benefit of future generations.

 

Conclusion

Deep-sea bioprospecting embarks on a journey into Earth’s ultimate uncharted realms, brimming with endless possibilities for future innovations in medicine, technology, and environmental preservation. This venture, teeming with the potential for discovering new pharmaceuticals, pioneering materials, and groundbreaking bioremediation methods, beckons us to unravel the mysteries hidden within the deep. However, as we navigate these uncharted waters, the importance of balanced exploration becomes increasingly clear. Protecting the delicate ecosystems of the deep sea is a crucial step in safeguarding its precious resources for the future.

 

Investing in deep-sea research is essential. It enables us to deepen our understanding of these complex habitats and their extraordinary residents, guiding us as we balance exploitation with conservation. The vast, uncharted depths of the deep sea present a rich resource for scientific discovery. As our exploration goes deeper, our commitment to a responsible path  is imperative, ensuring that the marvels of the deep are preserved and valued for generations to witness and cherish.

 

Be sure to visit bleedingedgebiology.com next week for another “bleeding edge” topic!

 

Further Exploration

Below is a list of resources that offer further exploration into the fascinating world of deep-sea bioprospecting, its ecosystems, and the technological and ethical considerations involved. This selection includes books, films, and articles, and TED talks providing a comprehensive look at various aspects of this topic.

 

Books

Films/Videos

  • “Aliens of the Deep” directed by James Cameron and Steven Quale (2005, Walt Disney Pictures): Cameron leads a team of scientists and marine biologists on an exploration of deep-sea hydrothermal vent ecosystems.
  • “Blue Planet II” (2017, BBC Earth): This documentary series, narrated by Sir David Attenborough, is dedicated to ocean exploration, including the deep sea, showcasing groundbreaking footage of weird sea creatures deep in the ocean.
  • “Ocean Watch: A Tale of Deep-Sea Exploration” (2024 Schmidt Ocean Institute) A 40-minute documentary film featuring new deep-sea discoveries and showcasing mesmerizing footage from Schmidt Ocean Institute and their new global-glass research vessel, R/V Falkor (too).

Articles

Ted Talks

  • “The deep sea’s medicinal secrets” by Sam Afoullouss 2021: Chemist Sam Afoullouss dives into the science behind natural remedies, explaining why the ocean’s great biodiversity is ideal for deriving and inspiring future medicinal treatments.
  • “Meet the mysterious ‘monsters’ of the deep sea” by Alan Jamieson 2021: The “aliens” of Earth live in the deepest parts of the ocean, and marine biologist Alan Jamieson has the photographs to prove it.
  • “The astonishing hidden world of the deep ocean”by Robert Ballard: Famous for discovering the Titanic, ocean explorer Robert Ballard discusses the technology enabling deep-sea exploration and the importance of uncovering the mysteries of the deep.
  • “Mining the Deep Sea”by Tom Peacock December 2019: This video discusses deep-sea mining of polymetallic nodules—an abundant new source of in-demand minerals, such as cobalt and nickel—to underpin the transition to a low carbon global economy.

Your Thoughts?

How do you see the future of deep-sea exploration shaping up? Are there specific areas within bioprospecting that intrigue you or concerns you have about the environmental impacts? Perhaps you have ideas about how we can better harness the ocean’s treasures while safeguarding its mysteries. Feel free to share your thoughts, questions, or any fascinating facts you’ve encountered about the deep sea.

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